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KMID : 0545120100200121681
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 12 p.1681 ~ p.1688
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Purification and Characterization of a Thermostable Cellobiohydrolase from Fomitopsis pinicola
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Shin Keum
Kim Yoon-Hee
Marimuthu Jeya
Lee Jung-Kul
Kim Yeong-Suk
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Abstract
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A screening for cellobiohydrolase (CBH) activity was performed and Fomitopsis pinicola KMJ812 was selected for further characterization as it produced a high level of CBH activity. An extracellular CBH was purified to homogeneity by sequential chromatography of F. pinicola culture supernatants. The molecular mass of the F. pinicola CBH was determined to be 64 kDa by SDS-PAGE and by size-exclusion chromatography, indicating that the enzyme is a monomer. The F. pinicola CBH showed a t1/2 value of 42 h at 70oC and catalytic efficiency of 15.8 mM-1 s-1 (kcat/ Km) for p-nitrophenyl-β-D-cellobioside, one of the highest levels seen for CBH-producing microorganisms. Its internal amino acid sequences showed a significant homology with hydrolases from glycoside hydrolase family 7. Although CBHs have been purified and characterized from other sources, the F. pinicola CBH is distinguished from other CBHs by its high catalytic efficiency and thermostability.
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KEYWORD
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cellobiohydrolase, enzyme production, Fomitopsis pinicola, glycoside hydrolase, purification, stability
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